Overview

Based on the draft genome sequence of Pacific bluefin tuna (PBT) Thunnus orientalis (Nakamura et al., 2013), we developed the first 44K PBT oligonucleotide microarray (oligo-array). Our work has been published in the journal Gene (Special issue: Marine Genomics) on February 1, 2016.

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Published paper

Motoshige Yasuike, Atushi Fujiwara, Yoji Nakamura, Yuki Iwasaki, Issei Nishiki, Takuma Sugaya, Akio Shimizu, Motohiko Sano, Takanori Kobayashi, Mitsuru Ototake (2016) A functional genomics tool for the Pacific bluefin tuna: Development of a 44K oligonucleotide microarray from whole-genome sequencing data for global transcriptome analysis. Gene. Volume 576,Issue 2, Part 1, Pages 603–609
(doi: 10.1016/j.gene.2015.10.023)

Abstract

Bluefin tunas are one of the most important fishery resources worldwide. Because of high market values, bluefin tuna farming has been rapidly growing during recent years. At present, the most common form of the tuna farming is based on the stocking of wild-caught fish. Therefore, concerns have been raised about the negative impact of the tuna farming on wild stocks. Recently, the Pacific bluefin tuna (PBT), Thunnus orientalis, has succeeded in completing the reproduction cycle under aquaculture conditions, but production bottlenecks remain to be solved because of very little biological information on bluefin tunas. Functional genomics approaches promise to rapidly increase our knowledge on biological processes in the bluefin tuna.

Here, we describe the development of the first 44K PBT oligonucleotide microarray (oligo-array), based on whole-genome shotgun (WGS) sequencing and large-scale expressed sequence tags (ESTs) data. In addition, we also introduce an initial 44K PBT oligo-array experiment using in vitro grown peripheral blood leukocytes (PBLs) stimulated with immunostimulants such as lipopolysaccharide (LPS: a cell wall component of Gram-negative bacteria) or polyinosinic:polycytidylic acid (poly I:C: a synthetic mimic of viral infection). This pilot 44K PBT oligo-array analysis successfully addressed distinct immune processes between LPS- and poly I:C- stimulated PBLs. Thus, we expect that this oligo-array will provide an excellent opportunity to analyze global gene expression profiles for a better understanding of diseases and stress, as well as for reproduction, development and influence of nutrition on tuna aquaculture production.

Contact Email

NRIFS - Tunas Genome Research Group
FRA-bioinfo@ml.affrc.go.jp